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mifM [2018-11-19 12:27:14]
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mifM [2018-11-19 12:27:14]

ribosome-nascent chain sensor of membrane protein biogenesis
locus
BSU23880
pI
5.83
mw
11.01 kDa
protein length
95 aa Sequence Blast
gene length
285 bp Sequence Blast
function
control of yidC2 translation
product
sensor of SpoIIIJ activity
essential
no
synonyms
yqzJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.12|Translation/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,483,586 → 2,483,873

    The protein

    Catalyzed reaction/ biological activity

  • acts as a kind of "leader peptide" that does or does not allow translation through a hairpin structure in the ''[gene|121A9543F5BC1CBE77D85A34099DE09F8AC63E9F|yidC2]'' mRNA [Pubmed|19779460]

    • [SW|Domains]

  • N-terminal transmembrane domain [Pubmed|22864117]
  • the C-terminal domain interacts with the ribosome and arrests the own translation elongation [Pubmed|22864117]

    • Structure

  • [PDB|3J9W] ([protein|C50AE06E8C959EB7176504C8E2BAD1A08E47C51A|MifM]-stalled [SW|ribosome] complex) [Pubmed|25903689]
  • [http://www.ebi.ac.uk/pdbe/entry/emdb/EMD-6306 EMD-6306] ([protein|C50AE06E8C959EB7176504C8E2BAD1A08E47C51A|MifM]-stalled [SW|ribosome] complex) [Pubmed|25903689]

    • [SW|Localization]

  • membrane [Pubmed|19779460]

    • Expression and Regulation

    Operons

    genes
    [gene|C50AE06E8C959EB7176504C8E2BAD1A08E47C51A|mifM]-[gene|121A9543F5BC1CBE77D85A34099DE09F8AC63E9F|yidC2]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|C50AE06E8C959EB7176504C8E2BAD1A08E47C51A|MifM]: attenuation, an mRNA hairpin of the yqjG transcript unfold upon translation arrest of [protein|C50AE06E8C959EB7176504C8E2BAD1A08E47C51A|MifM] at decreased [protein|D40C202E67A5319A91811DB11356F47A56C97DD2|YidC1] levels, in [regulon|C50AE06E8C959EB7176504C8E2BAD1A08E47C51A|MifM regulon]

    • regulation

  • repressed by large amounts of [SW|SpoIIIJ], induced by small amounts of [SW|SpoIIIJ] ([protein|search|MifM]) [Pubmed|19779460]

    • additional information

  • An [ncRNA|search|antisense RNA] is predicted for '[protein|search|mifM]' [PubMed|20525796]
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    Biological materials

    Mutant

  • BKE23880 (Δ[gene|C50AE06E8C959EB7176504C8E2BAD1A08E47C51A|mifM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACATCCATTCAATAGC, downstream forward: _UP4_TAAACCGCATTTATAAAAAG
  • BKK23880 (Δ[gene|C50AE06E8C959EB7176504C8E2BAD1A08E47C51A|mifM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACATCCATTCAATAGC, downstream forward: _UP4_TAAACCGCATTTATAAAAAG

    • References

  • 19779460,21383133,20525796,22456704,22383849,22864117,25313395,25903689